JHDM1D (KDM7A) Homogeneous Assay Kit

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Catalog #
50420
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Description

The JHDM1D Homogeneous Assay Kit is designed to measure JHDM1D (Jumonji C domain-containing histone demethylation protein 1D) activity for screening and profiling applications. The JHDM1D Homogeneous Assay Kit comes in a convenient 96-well or 384-well AlphaLISA® format, with enough purified recombinant JHDM1D (amino acids 2-488), substrate, primary antibody, assay and detection buffer for 100 or 400 enzyme reactions.

Figure 1: JHDM1D Homogenous Assay Kit schematic.
A sample containing JHDM1D is incubated with a biotinylated substrate. This is followed by the addition of acceptor beads and primary antibody, and finally donor beads. Alpha-counts are then counted. Alpha-counts are proportional to JHDM1D demethylase activity.

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Synonyms
Lysine-specific demethylase 7A, KDM7A, KIAA1718
Product Info
Storage and Usage
Citations
Assay Kit Format
AlphaLISA®
Materials Required But Not Supplied
  •  AlphaLISA® anti-rabbit IgG acceptor beads, 5 mg/ml (Perkin Elmer #AL104C)
  •  AlphaScreen® Streptavidin-conjugated donor beads, 5 mg/ml (Perkin Elmer #6760002S)
  •  Optiplate - 384 (Perkin Elmer #6007290)
  •  AlphaScreen® microplate reader
Format

96 reactions

Catalog # Name Amount Storage
50419 JHDM1D (KDM7A), FLAG-Tag* 5 µg -80°C
52140Q4 Primary Antibody 17-4 5 µl -80°C
79845 Biotinylated Histone H3 Peptide Substrate 300 reactions -80°C
52409 4x HDM Assay Buffer 4 3 x 1 ml -80°C
52301 4x Detection Buffer 2 ml -20°C

*The initial concentration of enzyme is lot-specific and will be indicated on the tube containing the protein.

 

384 reactions

Catalog # Name Amount Storage
50419 JHDM1D (KDM7A), FLAG-Tag* 20 µg -80°C
52140Q4 Primary Antibody 17-4 20 µl -80°C
79845 Biotinylated Histone H3 Peptide Substrate 500 reactions -80°C
52409 4x HDM Assay Buffer 4 3 x 1 ml -80°C
52301 4x Detection Buffer 2 ml -20°C

*The initial concentration of enzyme is lot-specific and will be indicated on the tube containing the protein.

UniProt #
A0JNV9
Background

JHDM1D (Jumonji C domain-containing histone demethylation protein 1D), also known as KIAA1718 and KDM7A (lysine demethylase 7A), is a JumonjiC (JmjC) domain containing histone lysine demethylase with demethylation activity toward H3-K9Me2 and H3-K27Me2. They are Fe(II) and α-ketoglutarate (2OG) dependent, producing carbon dioxide, succinate and unmethylated protein. By demethylating H3-K9Me2 and H3-K27Me2, which are inhibitory when in their methylated form, JHDM1D releases the brake on transcription and allows for expression of genes involved in multiple biological processes, namely embryonic development, neurogenesis and lipid metabolism. It is linked to tumorigenesis, immune related diseases and neurological and developmental disorders. Multiple inhibitors of JHDM1D inhibitors have been developed, aimed at cancer and osteoarthritis treatment. Drug development targeting JHDM1D is an area of interest, and the development of specific inhibitors will be beneficial in the cancer therapy field.

References

Horton, J.R., et al., 2010 Nature Structural & Molecular Biology; 17(1): 38-43.
Li C., et al., 2023 Biochemical Pharmacology 216: 115799.