JHDM1D (KDM7A) Homogeneous Assay Kit

Catalog #: 50420
Size: 384 reactions
$1,725
Description:
The JHDM1D Homogeneous Assay Kit is designed to measure activity of JHDM1D for screening and profiling applications. JHDM1D, also known as KDM7A and KIAA1718, is a JumonjiC (JmjC) domain-containing histone lysine demethylase that exhibits demethylation activity toward dimethyl-lysine 9 and dimethyl-lysine 27 on histone H3 (H3K9me2 and H3K27me2 respectively). The key to the JHDM1D Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, a sample containing JHDM1D enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
UniProt
A0JNV9
Synonym(s):
Lysine-specific demethylase 7A, KDM7A, KIAA1718, AlphaLISA, Alphascreen, Alpha Lisa, Alpha screen
Supplied As:
The JHDM1D Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JHDM1D for 384 enzyme reactions.
Contraindications:
Green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of culture medium RPMI 1640 at >1% leads to signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays.
Format:
Instructions for use:
See assay kit datasheet for detailed protocol.
Storage / Stability:
At least 6 months from date of receipt when stored as directed.
Application(s):
Great for studying enzyme kinetics and HTS applications.
Reference(s):
Horton J.R., et al. Nature Structural & Molecular Biology 2010; 17(1):38-43.
Notes:
AlphaScreen® and AlphaLISA® are registered trademarks of PerkinElmer, Inc.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Demethylase
Product Type:
Assay Kit
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Product Datasheets

50420_Lot_170712.pdf