JHDM1D (KDM7A) Chemiluminescent Assay Kit
The JHDM1D Chemiluminescent Assay Kit is designed to measure JHDM1D activity for screening and profiling applications. JHDM1D, also known as KDM7A and KIAA1718, is a JumonjiC (JmjC) domain-containing histone lysine demethylase that exhibits demethylation activity toward dimethyl-lysine 9 and dimethyl-lysine 27 on histone H3 (H3K9me2 and H3K27me2 respectively). The key to the JHDM1D Chemiluminescent Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for detection of demethylase activity. First, JHDM1D enzyme is incubated with the methylated H3 peptide for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.
| Catalog # | Component | Amount | Storage | |
| 50419 | JHDM1D* | 5 μg | -80°C |
Avoid freeze/ |
| 52140Q3 | Primary antibody 17-4 | 12.5 μl | -80°C | |
| 52131H | Secondary HRP-labeled antibody 2 | 10 μl | -80°C | |
| 4x JHDM1D direct assay buffer | 3 x 1 ml | -80°C | ||
| 52100 | Blocking buffer 4 | 50 ml | +4°C | |
| 79670 | ELISA ECL substrate A (transparent bottle) | 6 ml | Room Temp | |
| ELISA ECL substrate B (brown bottle) | 6 ml | Room Temp | ||
| White 8-well strip plate precoated with histone substrate | 1 | +4°C | ||
*The concentration of JHDM1D is lot-specific and will be indicated on the tube containing the enzyme.
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