JARID1B (KDM5B) TR-FRET Assay Kit

Catalog #: 50514
Size: 384 reactions
$1,995
Background:
JARID1B, also known as PLU-1 and KDM5B, is a JumonjiC (JmjC) and ARID domain-containing histone lysine demethylase that exhibits demethylation activity toward di- and trimethyl-lysine 4 (H3K4me2/3) on histone H3.
Description:
The JARID1B TR-FRET Assay Kit is designed to measure activity of JARID1B in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The JARID1B TR-FRET Assay Kit comes in a convenient format, with histone H3 peptide substrate, a Eu-labeled antibody against methylated K4 residue of Histone H3, demethylase assay buffer, TR-FRET detection buffer, dye-labeled acceptor, and purified JARID1B for 384 enzyme reactions. The key to the JARID1B Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, a sample containing JARID1B enzyme is incubated with the biotinylated substrate. Next, antibody is added. Finally, dye-labeled acceptor is added followed by fluorescence reading.
Synonym(s):
JARID1B, PLU-1, KDM5B, HTRF
Format:

COMPONENTS:

Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:
At least 6 months from date of receipt when stored as directed.
Application(s):
Great for screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s):
Lahoud, M.H., et al. Genome Res. 11 (8): 1327–34.
Notes:
MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Demethylase
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

50514.pdf