The JARID1B TR-FRET Assay Kit is designed to measure activity of JARID1B
in a homogeneous 384 reaction format. This FRET-based
assay requires no time-consuming washing steps, making it especially suitable for high
throughput screening applications. The JARID1B TR-FRET Assay Kit comes in a convenient
format, with histone H3 peptide substrate, a Eu-labeled antibody against methylated K4 residue
of Histone H3, demethylase assay buffer, TR-FRET detection buffer, dye-labeled acceptor, and
purified JARID1B for 384 enzyme reactions. The key to the JARID1B Assay Kit is a highly
specific antibody that recognizes demethylated substrate. With this kit, only three simple steps
on a microtiter plate are required for demethylase activity detection. First, a sample containing
JARID1B enzyme is incubated with the biotinylated substrate. Next, antibody is added. Finally,
dye-labeled acceptor is added followed by fluorescence reading.
JARID1B, also known as PLU-1 and KDM5B, is a JumonjiC (JmjC) and ARID domain-containing histone lysine demethylase that exhibits demethylation activity toward di- and trimethyl-lysine 4 (H3K4me2/3) on histone H3.
References
Lahoud, M.H., et al. Genome Res. 11 (8): 1327–34.
Storage/Stability
At least 6 months from date of receipt when stored as directed.
Instructions for Use
See assay kit data sheet for detailed protocol.
Applications
Useful for screening small molecular inhibitors for drug discovery and HTS applications.
Shipping Temperature
Dry ice
Notes
MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED: Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Adjustable micropipettor and sterile tips
Warnings
Avoid freeze/thaw cycles.
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.
Powered by Bioz
