JARID1B (KDM5B) Chemiluminescent Assay Kit

Catalog #

50517

$725 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The JARID1B Chemiluminescent Assay Kit is designed to measure JARID1B activity for screening and profiling applications. JARID1B is a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. It binds directly, with several other proteins, to retinoblastoma protein which regulates cell proliferation. The key to the JARID1B Chemiluminescent Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for detection of demethylase activity. First, JARID1B enzyme is incubated with the methylated H3 peptide for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

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Synonyms

JARID1B, PLU-1, KDM5B, Retinoblastoma-Binding Protein 2 Homolog 1, RBP2-H1, Jumonji, AT Rich Interactive Domain 1B

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Supplied As

The JARID1B Chemiluminescent Assay Kit comes in a convenient format, with a 96-well strip plate pre-coated with methylated histone H3 peptide substrate, primary antibody, secondary HRP-labeled antibody, demethylase assay buffer, and purified JARID1B.

Format

Catalog Number	Component	Amount	Storage
50121	JARID1B (KDM5B, PLU-1)	20 µg	-80°C	*Avoid freeze/ thaw cycles!*
52140M	Primary antibody 13	12.5 µl	-80°C
52130H	Secondary HRP-labeled antibody 1	10 µl	-80°C
	4x JARID1B assay buffer	3 x 1 ml	-80°C
52100	Blocking buffer 4	50 ml	+4°C
79670	ELISA ECL substrate A (transparent bottle)	6 ml	Room Temp.
79670	ELISA ECL substrate B (brown bottle)	6 ml	Room Temp.
	8-well strip plate module pre-coated with histone substrate	1	+4°C

UniProt #

Q9UGL1

References

Klose, R.J., et al. Cell 2007; 128: 889.

Storage/Stability

6 months from date of receipt when stored as directed.

Instructions for Use

See assay kit data sheet for detailed protocol

Applications

Useful for studying enzyme kinetics and HTS applications.

Shipping Temperature

-80°C (dry ice)

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Demethylase

Regulatory Status

Research Use Only