JMJD3 Homogeneous Assay Kit
Catalog #50416

Size: 384 reactions

DESCRIPTION:
The JMJD3 Homogeneous Assay Kit is designed to measure activity of the JMJD3 for screening
and profiling applications. JMJD3 is a JmjC-domain protein that exhibits demethylation activity
toward di- and trimethyl-lysine 27 (H3K27me2/3) on histone H3. The JMJD3 Homogeneous Assay
Kit comes in a convenient AlphaLISA® format, with biotinylated histone H3 peptide substrate,
primary antibody, demethylase assay buffer, and purified JMJD3 for 384 enzyme reactions. The
key to the JMJD3 Homogeneous Assay Kit is a highly specific antibody that recognizes
demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for
methyltransferase detection. First, a sample containing JMJD3 enzyme is incubated with the
biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads,
followed by reading the Alpha-counts.

COMPONENTS:

APPLICATIONS: Great for studying enzyme kinetics and HTS applications.

STABILITY: At least one year from date of receipt when stored as directed.

REFERENCE: Swigut T., Wysocka J. Cell 2007; 131(1): 29-32.

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