JMJD3 Chemiluminescent Assay Kit
Catalog # 50406
Size: 96 reactions
DESCRIPTION: The JMJD3 Chemiluminescent Assay Kit is designed to measure JMJD3
activity for screening and profiling applications. JMJD3 is a JmjC-domain protein that
exhibits demethylation activity toward di- and trimethyl-lysine 27 (H3K27me2/3) on histone
H3. The JMJD3 Assay Kit comes in a convenient format, with 8-well strips precoated with
the methylated histone H3 peptide substrate, primary antibody, the secondary HRPlabeled
antibody, demethylase assay buffer, and purified JMJD3 for 100 enzyme reactions.
The key to the JMJD3 Direct Activity Assay Kit is a highly specific antibody that recognizes
demethylated substrate. With this kit, only three simple steps on a microtiter plate are
required for methyltransferase detection. First, a sample containing JMJD3 enzyme is
incubated with a sample containing assay buffer for one hour. Next, primary antibody is
added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by
addition of the HRP substrate to produce chemiluminescence that can then be measured
using a chemiluminescence reader.
APPLICATIONS: Great for studying enzyme kinetics and HTS applications.
STABILITY: One year from date of receipt when stored as directed.
REFERENCE: Swigut T, Wysocka J. Cell 2007; 131(1): 29.