JARID1A Homogeneous Assay Kit
Catalog # 50510
Size: 384 reactions
The JARID1A Homogeneous Assay Kit is designed to measure activity of the JARID1A for
screening and profiling applications. JARID1A, also known as RBBP2 and KDM5A, is a JumonjiC
(JmjC) and ARID domain-containing histone lysine demethylase that exhibits demethylation
activity toward di- and trimethyl-lysine 4 (H3K4me2/3) on histone H3. The JARID1A
Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with biotinylated histone H3
peptide substrate, primary antibody, demethylase assay buffer, and purified JARID1A for 384
enzyme reactions. The key to the JARID1A Homogeneous Assay Kit is a highly specific antibody
that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate
are required for demethylase activity detection. First, a sample containing JARID1A enzyme is
incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added,
then donor beads, followed by reading the Alpha-counts.
APPLICATIONS: Great for studying enzyme kinetics and HTS applications.
STABILITY: At least one year from date of receipt when stored as directed.
REFERENCE: DiTacchio L, Le HD, Vollmers C et al. Science 2011; 333(6051):1881-5.