Sirtuin 1 (193-741), GST-tag

Catalog #: 50012
Size: 100 µg
$425
Species: Human
Host Species: E. coli
MW: 88 kDa
Genbank #: NM_012238
Tag(s): N-terminal GST-tag
a.a: 193– 741
Description:
Human Sirtuin 1 , GenBank Accession No. NM_012238, a.a. 193-741 with N-terminal GST-tag, MW= 88 kDa, expressed in an E. coli expression system.
UniProt
Q96EB6
Synonym(s):
Sirtuin1, SIRT1, Sir2, Sir2a
Specific Activity:
≥20 pmol/min /µg
Assay Conditions:
25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate 1 (Catalog #50032), 500 µM NAD+ and Sirtuin 1 (12.5 - 800 ng). Incubation condition: 30 min at 37°C followed by treatment with 50 µl of SIRT developer (Catalog # 50089) for 15 min at RT. Fluorescence intensity is measured at ex360/em460.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Warning(s):
Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).
Scientific Category:
Deacetylase
Product Type:
Recombinant Protein
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Product Datasheets

50012.pdf