pf-HDAC1, Malaria, His-tag, FLAG-tag

Catalog #: 50063
Size: 20 µg
$425
Species: Malaria (Plasmodium falciparum)
Host Species: Sf9 insect cells
MW: 53.4 kDa
Genbank #: AAD22407.1
Tag(s): C-terminal His-tag and FLAG-tag
a.a: 1–450
Description:
Malarial (Plasmodium falciparum) HDAC, Genbank Accession No. AAD22407.1, full length (1-450) with C-terminal His-tag and FLAG-tag, MW=53.4 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
Q7K6A1
Synonym(s):
histone deacetylase 1 plasmodium, falciparum, pf-HDAC-1 Malaria
Specific Activity:
≥1.85 pmol/min/µg
Assay Conditions:
25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate (Cat. #50037), and 2 ng/µl pfHDAC. HDAC reaction was performed for 30 min at 37°C followed by treatment with 50 µl of HDAC developer (Cat. #50030) for 15 min at RT. Fluorescence intensity is measured at ex360/em460.
Formulation:
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 µg/ml FLAG peptide, 10% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Chen et al., J. Med. Chem. 2008 Jun 26; 51(12):3437-48.
2. Joshi et al., Mol. Biochem. Parasitol. 1999 Mar 15; 99(1):11-9.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Deacetylase
Product Type:
Recombinant Protein
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Product Datasheets

50063_Lot100412.pdf