HDAC9, His-tag

Catalog #: 50009
Size: 10 µg
$425
Species: Human
Host Species: Sf9 insect cells
MW: 50.7 kDa
Genbank #: NM_178423
Tag(s): C-terminal His-tag
a.a: 604–1066
Description:
Human HDAC9 , GenBank Accession No. NM_178423, a.a. 604-1066 with C-terminal His-tag, MW= 50.7 kDa, expressed in baculovirus expression system.
UniProt
Q9UKV0
Synonym(s):
histone deacetylase 9, HDAC-9
Specific Activity:
≥3000 pmol/min/µg
Assay Conditions:
25 mM Tris HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC class 2a substrate (Catalog #50040), and HDAC9 (0.15 - 10 ng). Incubation condition: 30 min at 37°C, followed by HDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 200 mM imidazole
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Deacetylase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

50009_Lot130502-66.pdf