HDAC6 (H611A), GST-tag

Catalog #: 50066
Size: 50 µg
$450
Species: Human
Host Species: Sf9 insect cells
MW: 161 kDa
Genbank #: NM_006044
Tag(s): N-terminal GST-tag
a.a: full length
Description:
Human HDAC6 with H611A mutation (GenBank Accession No. NM_006044), full length with N-terminal GST-tag, MW= 161 kDa, expressed in a baculovirus expression system.
UniProt
Q9UBN7
Synonym(s):
histone deacetylase 6, HDAC6
Specific Activity:
2.5 pmole/min/µg

Assay Conditions:
25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate 3 (Catalog #50037), and HDAC6 H611A (15.6 - 1000ng). Incubation condition: 30 min at 37°C followed by HDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.
Formulation:
50 mM Tris-HCl, pH 7.5, 500 mM NaCl, 50 mM KCl, 2 mM EDTA, 10% glycerol, and 2 mM DTT
Purification:
Protein was purified by affinity chromatography and gel filtration.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Deacetylase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

50066_Lot140707.pdf