HDAC6 (H611A), GST-tag

Catalog #: 50066
Size: 50 µg
$450
Species: Human
Host Species: Sf9 insect cells
MW: 161 kDa
Genbank #: NM_006044
Tag(s): N-terminal GST-tag
a.a: full length
Description:
Human HDAC6 with H611A mutation (GenBank Accession No. NM_006044), full length with Nterminal GST tag, MW= 161 kDa, expressed in a baculovirus expression system.
UniProt
Q9UBN7
Synonym(s):
histone deacetylase 6, HDAC6
Specific Activity:
0.83 pmole/min/µg
Assay Conditions:
Assay Conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate 3 (Catalog #50037), and HDAC6 H611A (15 – 1000 ng). Incubation condition: 30 min at 37ºC followed by developing for 15 min at room temperature.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20 and 20% Glycerol.
Purification:
Protein was purified by affinity chromatography and gel filtration.
Format:
Aqueous buffer solution
Storage / Stability:
At least 6 months at –80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Warning(s):

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Scientific Category:
Deacetylase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

50066_lot_171215-G.pdf