HDAC6, GST-Tag (Mouse)

Catalog #: 50057
Size: 50 µg
$425
Species: Mouse
Host Species: Sf9 cells
MW: 152 kDa
Genbank #: NM_010413
Tag(s): N-terminal GST tag
a.a: 2-1149 (end)
Description:
Mouse HDAC6, also known as histone deacetylase 6, GenBank Accession No. NM_010413, a.a. 2-1149 (end) with N-terminal GST-Tag, expressed in baculovirus expression system. MW = 152 kDa.
Synonym(s):
histone deacetylase 6, HDAC 6, HDAC-6
Assay Conditions:
25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate 3 (Catalog #50037), and Mouse HDAC6 (3.1 – 200 ng). Incubation condition: 30 min at 37ºC followed by developing for 15 min at room temperature.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol
Storage / Stability:
At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Rikimaru, T. et al., Oncology 72(1-2), 69-74 (2007).

2. Ishihama, K. et al., J Clin Pathol 60(11), 1205-10 (2007).

Scientific Category:
HDAC
Product Type:
Recombinant Protein
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Product Datasheets

50057 Lot 170110-2.pdf