HDAC6, GST-Tag (HEK293-derived)

Catalog #: 100051
Size: 50 µg
$425
Species: Human
Host Species: HEK293
MW: 161 kDa
Genbank #: NM_006044
Tag(s): N-terminal GST tag
a.a: 1-1215
Description:
Human HDAC6, a.a. 1-1215, N-terminal GST tag, GenBank Accession No. NM_006044, expressed in a HEK293 expression system. MW = 161 kDa.
Specific Activity:
300 pmol/min/µg
Assay Conditions:
25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate 3 (Catalog #50037), and HDAC6 (3.2 – 200ng). Incubation condition: 30 min at 37ºC followed by developing for 15 min at room temperature.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 3mM DTT
Storage / Stability:
At least 12 months at –80°C
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Li S. et al., Neurology. 41(2), 112-6 (2010).

2. Strausberg, R.L. et al., Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002).

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
HDAC
Product Type:
Protein
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Product Datasheets

100051_Lot_180508-2.pdf