HDAC6, FLAG-tag

Catalog #: 50056
Size: 50 µg
$425
Species: Human
Host Species: Sf9 insect cells
MW: 132 kDa
Genbank #: NM_006044
Tag(s): C-terminal FLAG-tag
a.a: full length
Description:
Human HDAC6 (GenBank Accession No. NM_006044), full length with C-terminal FLAG-tag, MW= 132 kDa, expressed in baculovirus expression system.
UniProt
Q9UBN7
Synonym(s):
histone deacetylase 6, HDAC-6
Specific Activity:
≥146 pmol/min/µg.
Unit Definition:
One U =1 pmol of acetyl group removed/min/µg of enzyme
Assay Conditions:
25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 30 µM BPS HDAC substrate (Catalog #50037), and HDAC6. Incubation condition: 30 min at 37°C followed by HDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ng/µl FLAG peptide,and 20% glycerol.
Purification:
Protein was purified by affinity chromatography and gel filtration.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Deacetylase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

50056_141007.pdf