HDAC5 (full length), GST-tag

Catalog #: 50045
Size: 5 µg
$450
Species: Human
Host Species: Sf9 insect cells
MW: 150 kDa
Genbank #: NM_005474
Tag(s): N-terminal GST-tag
a.a: full length
Description:
Human HDAC5 , GenBank Accession No. NM_005474, full length with N-terminal GST-tag, MW= 150 kDa, expressed in baculovirus expression system.
UniProt
Q9UQL6
Synonym(s):
histone deacetylase 5, HDAC-5
Unit Definition:
One U =1 pmol of acetyl group removed/min/µg of enzyme
Assay Conditions:
25 mM Tris HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS substrate(Catalog number 50040), and 0.8-50 ng/µl HDAC5. Incubation condition: 30 min at 37°C followed by HDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Deacetylase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

50045_Lot130620.pdf