HDAC5, Catalytic Domain, His-tag

Catalog #: 50005
Size: 10 µg
$425
Species: Human
Host Species: Sf9 insect cells
MW: 51 kDa
Genbank #: NM_005474
Tag(s): C-terminal His-tag
a.a: 656–1122 (end)
Description:
Human HDAC5 catalytic domain , GenBank Accession No. NM_005474, a.a. 656-1122 (end) with C-terminal His-tag, MW= 51 kDa, expressed in baculovirus expression system.
UniProt
Q9UQL6
Synonym(s):
histone deacetylase 5, HDAC-5
Specific Activity:
≥1000 pmol/min/µg.
Unit Definition:
One U =1 pmol of acetyl group removed/min/µg of enzyme
Assay Conditions:
25 mM Tris HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 30 µM BPS HDAC class 2a substrate , BPS Catalog number 50040, and HDAC5. Incubation condition: 30 min at 30°C, followed by HDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.
Formulation:
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 imidazole, and 10% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Deacetylase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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