HDAC10, GST-tag, His-tag

Catalog #: 50010
Size: 50 µg
$425
Species: Human
Host Species: Sf9 insect cells
MW: 78 kDa
Genbank #: NM_032019
Tag(s): N-terminal GST-tag, C-terminal His-tag
a.a: 1–481
Description:
Human HDAC10 (a.a. 1-481), GenBank Accession No. NM_032019, with N-terminal GST-tag amd C-terminal His-tag, MW= 78 kDa, expressed in baculovirus expression system.
UniProt
Q969S8
Synonym(s):
histone deacetylase 10, HDAC10
Specific Activity:
≥4.4 pmol/min/µg.
Assay Conditions:
25 mM Tris HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate 3 (Catalog #50037), and HDAC10 (15.6 - 2000 ng). Incubation condition: 30 min at 37°C, followed by HDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Deacetylase
Product Type:
Recombinant Protein
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Product Datasheets

50010_lot140423.pdf