Deacetylases Assay Kits

This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of other assays. Only two simple steps on a microtiter plate are needed to analyze HDAC activity. First, an HDAC fluorometric substrate is incubated with purified HDAC enzyme. The deacetylation catalyzed by HDAC sensitizes the resulting product to subsequent treatment with the developer releases a fluorophore that is easily measured on a fluorescence reader.

  
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