SIRT5 (Sirtuin5) Fluorogenic Assay Kit

Catalog #
50085
$465 *
Size: 96 reactions
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Description

The Fluorogenic SIRT5 Assay Kit is a complete assay system designed to measure SIRT5 activity for screening and profiling applications. The Fluorogenic SIRT5 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only three simple steps on a microtiter plate are needed to analyze the SIRT5 activity level. First, the Sirtuin 5 fluorometric substrate (SIRT substrate 5), containing a succinylated lysine side chain, is incubated with purified SIRT5 enzyme. The desuccinylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.

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This product has been cited 3 times.

Synonyms
sirt5, sirtuin 5
Product Info
Storage and Usage
Citations3
Assay Kit Format
Fluorogenic
Supplied As
The Fluorogenic SIRT5 Assay Kit comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SIRT5 activity measurements.
Format

Assay Kit Components

UniProt #
Q9NXA8
References
1.J. Du et al. (2011) Science. 334: 806.
2. A. Madsen et al. (2012) J. Med. Chem 55: 5582.
3. F. Fisher et al. (2012) Plos One. 7: 1.