VEGF165, Mouse

Catalog #: 91000-1
Size: 10 µg
Price: $195
Species: Mouse
Host Species: E. coli
MW: 39 kDa
Genbank #: NM_009505
Tag(s): none
a.a: 27–190
Description:
Recombinant mouse VEGF (GenBank Accession No. NM_009505) is a 39 kDa disulfide-linked homodimeric protein (a.a. 27-190), consisting of two 164 amino acid polypeptide.
UniProt
Q00731
Synonym(s):
Vascular endothelial growth factor 165, VEGF
Purity:
≥98% by SDS-PAGE and HPLC analysis.
Endotoxin Level:
< 1EU per µg of VEGF as determined by the LAL method.
Biological Activity:
ED50 is less than 2 ng/ml as determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC).
Formulation:
Lyophilized from 0.2 µm filtered 20 mM PBS solution, pH 7.0
Format:
lyophilized protein
Reconstitution:
Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage / Stability:

≥2 years (lyophilized) at -20°C. Reconstituted solutions can be stored in undiluted aliquots at 4°C for one week or at -20°C for up to six months.

Application(s):
Useful for cell culture and for the study of signaling and developmental pathways. Also useful for receptor binding studies, screening inhibitors, and selectivity profiling.
Reference(s):
1. Lee, S., et al. Cell 130 (4), 691-703 (2007)
2. Yamamoto, H., et al. Dev. Biol. 308 (1), 44-53 (2007)
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Cytokine
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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