VEGF 165 (human, recombinant)

Catalog #: 91001-2
Lot# 110413

Size: 20 µg

Description: Recombinant human VEGF165 (GenBank Accession No. NM_001025368) is a 38.2
kDa disulfide-linked homodimeric protein (a.a. 207-371), consisting of two 165 amino acid
polypeptide.

Source: E.coli

Stability: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C.

Purity: >98% by SDS-PAGE and HPLC analysis.

Endotoxin Level: < 1EU per µg of VEGF as determined by the LAL method.

Biological Activity: ED50 is less than 3 ng/ml as determined by the dose-dependent stimulation
of the proliferation of human umbilical vein endothelial cells (HUVEC).

STORAGE/HANDLING:
The lyophilized powder, though stable at room temperature, is best
stored at -20°C. After a quick spin, reconstitute with sterile 4 mM
HCl to a concentration of 0.1-1.0 mg/mL. This solution can be
diluted into water or other buffered solutions and stored at 4°C for
one week or at -20°C for future use. Reconstituted human VEGF
should be stored in undiluted aliquots at -20°C for up to six months.

AA SEQUENCE:
APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPD
EIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKP
HQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKH
LFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

References:
1. VEGF serum levels are not associated with Parkinson's disease Eur. J. Neurol. 14 (8), E6
(2007)
2. Association between vascular endothelial growth factor gene polymorphisms and
survival in hepatocellular carcinoma patients Hepatology 46 (2), 446-455 (2007)

PRODUCT DATA SHEETS
Lot 110413

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