EGF, Mouse

Catalog #: 90200-1
Size: 100 µg
Price: $75
Species: Mouse
Host Species: E. coli
MW: 6.0 kDa
Genbank #: NM_010113
Tag(s): none
a.a: 977– 1029
Description:
Mouse Epidermal Growth Factor (GenBank Accession No. NM_010113), a.a. 977- 1029, MW=6.0 kDa, expressed in an E. coli expression system.
Synonym(s):
epidermal growth factor, EGF, beta-urogastrone
Purity:
≥98% by SDS-PAGE and HPLC analysis.
Endotoxin Level:
< 1EU per µg of EGF as determined by the LAL method.
Biological Activity:
ED50 is 0.1 - 0.2 ng/ml as determined by a cell proliferation assay using BALB/c 3T3 cells.
Formulation:
Lyophilized  from  a  sterile  filtered  solution  containing  10  mM  Phosphate Buffered Saline (PBS), pH 7.4, 138 mM NaCl, and 2.7 mM KCl.
Format:
lyophilized protein
Reconstitution:
Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage / Stability:

≥2 years (lyophilized) at -20°C.

Application(s):
Useful for cell culture and for the study of signaling pathways. Also useful for receptor binding studies, screening inhibitors, and selectivity profiling.
Reference(s):
1. Michaylira, C.Z., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 291 (3), G472-G481
2. Duncan, E.A., et al. J. Biol. Chem. 281 (18), 12521-12525 (2006)
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Cytokine
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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