EGF, Mouse

Catalog #: 90200-1
Size: 100 µg
Price: $75
Species: Mouse
Host Species: E. coli
MW: 6.0 kDa
Genbank #: NM_010113
Tag(s): none
a.a: 977– 1029
Mouse Epidermal Growth Factor (GenBank Accession No. NM_010113), a.a. 977- 1029, MW=6.0 kDa, expressed in an E. coli expression system.
epidermal growth factor, EGF, beta-urogastrone
≥98% by SDS-PAGE and HPLC analysis.
Endotoxin Level:
< 1EU per µg of EGF as determined by the LAL method.
Biological Activity:
ED50 is 0.1 - 0.2 ng/ml as determined by a cell proliferation assay using BALB/c 3T3 cells.
Lyophilized  from  a  sterile  filtered  solution  containing  10  mM  Phosphate Buffered Saline (PBS), pH 7.4, 138 mM NaCl, and 2.7 mM KCl.
lyophilized protein
Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage / Stability:

≥2 years (lyophilized) at -20°C.

Useful for cell culture and for the study of signaling pathways. Also useful for receptor binding studies, screening inhibitors, and selectivity profiling.
1. Michaylira, C.Z., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 291 (3), G472-G481
2. Duncan, E.A., et al. J. Biol. Chem. 281 (18), 12521-12525 (2006)
Avoid freeze/thaw cycles.
Scientific Category:
Product Type:
Recombinant Protein
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