EGF

Catalog #: 90201-1
Size: 100 µg
$85
Species: Human
Host Species: E. coli
MW: 6.2 kDa
Genbank #: NM_001963
Tag(s): none
a.a: 971– 1023
Description:
Human Epidermal Growth Factor (GenBank Accession No. NM_001963), a.a. 971- 1023, MW=6.2 kDa, expressed in an E. coli expression system.
UniProt
P01133
Synonym(s):
epidermal growth factor, EGF, beta-urogastrone
Purity:
≥98% by SDS-PAGE and HPLC analysis.
Endotoxin Level:
< 1EU per µg of EGF as determined by the LAL method.
Biological Activity:
ED50 is < 0.1 ng/ml. ED50 was determined by a cell proliferation assay using BALB/c 3T3 cells
Formulation:
Lyophilized  from  a  sterile  filtered  solution  containing  10  mM  Phosphate Buffered Saline (PBS), pH 7.4, 138 mM NaCl, and 2.7 mM KCl.
Format:
lyophilized protein
Reconstitution:
Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage / Stability:

≥2 years (lyophilized) at -20°C.

Application(s):
Useful for cell culture and for the study of signaling pathways. Also useful for receptor binding studies, screening inhibitors, and selectivity profiling.
Reference(s):
1. Su, X., et al. J. Biol. Chem. 282 (29), 21278-21284 (2007)
2. Jin, G., et al. Cancer Sci. 98 (6), 864- 868 (2007)
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Cytokine
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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