Basic FGF/FGF2

Catalog #: 90300-4
Size: 50 µg
$210
Species: Human
Host Species: E. coli
MW: 17.3 kDa
Genbank #: NM_002006
Tag(s): none
a.a: 134 – 288
Description:
Recombinant human soluble basic FGF (GenBank Accession No. NM_002006) is a 17.3 kDa (134 aa-288 aa) protein expressed in an E. coli expression system.
UniProt
P09038
Synonym(s):
Basic Fibroblast Growth Factor , FGF2, bFGF
Purity:
≥98% by SDS-PAGE and HPLC analysis.
Endotoxin Level:
< 1EU per ug of bFGF as determined by the LAL method.
Biological Activity:
Measured by its ability to stimulate proliferation of NIH3T3 cells. The ED50 of this effect is between 0.1 - 1 ng/ml
Formulation:
Lyophilized from a 0.2 µm filtered solution containing 5 mM Tris, pH 7.5, 150 mM NaCl
Format:
lyophilized protein
Reconstitution:
Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage / Stability:

≥2 years (lyophilized) at -20°C.

Application(s):
Useful for cell culture and for the study of signaling pathways.
Reference(s):
1. Matsui, Y., et al. Cell 70(5), 841-847 (1992).
2. Segev, H, et al. Stem Cells. 22(3), 265-74 (2002).
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Cytokine
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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