Human BAFF-R(CD268)

Catalog #: 90103-A
Size: 10 µg
$85
Species: Human
Host Species: E. coli
MW: 9 kDa
Genbank #: Q96RJ3
a.a: 1–78
Background:
The B cell-activating factor from the TNF family (BAFF), is emerging as an important regulator of B cell and T cell responses. BAFF was originally identified as a factor responsible for B cell survival and maturation .BAFF binds to several receptors. These include transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI), BAFF-R (BR3), and B cell maturation Ag (BCMA). BAFF-R appears to be particularly important for the regulation of B cell survival and maturation in the spleen, because A/WySnJ mice expressing a defective BAFF-R have disrupted B cell maturation, similar to that seen in BAFF-deficient mice.
Description:
Recombinant human BAFF-R (B cell-activating factor from the TNF family), also known as CD268, is a disulfide-linked monomeric protein consisting of 76 a.a. and migrates as an approximately 9 kDa protein under reducing and non-reducing conditions. Optimized DNA sequence encoding human BAFF-R extracellular domain was expressed in E. coli.
UniProt
Q96RJ3
Synonym(s):
BAFF-R, B cell-activating factor from the TNF family, CD268
Purity:
≥95% by SDS-PAGE and HPLC
Endotoxin Level:
<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Biological Activity:
The ED50 , determined by the ability to block BAFF induced survival of splenocyte cells, was found to be in the range of 1.0-5.0 µg/ml.
Formulation:
Lyophilized from a 0.2 µm filtered solution containing 2.5% glycine, 0.5% sucrose, 0.01% Tween-80, 5 mM glutamic acid, pH 4.5.
Format:
lyophilized protein
Reconstitution:
Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage / Stability:

The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.

Reference(s):
1. The effects of BAFF and BAFF-R-fc fusion protein in immune thrombocytopenia. Blood, Dec 2009, 114: 5362 - 5367.
2. c-rel in the regulation of BAFF-R expression in malignant human B cells. Blood (ASH Annual Meeting Abstracts), Nov 2009, 114: 2400.
Warning(s):
Avoid freeze/thaw cycles
Amino Acid Sequence:
MRRGPRSLRG RDAPAPTPCV PAECFDLLVR HCVACGLLRT PRPKPAGASS PAPRTALQPQ ESVGAGAGEA ALPLPGLL
Scientific Category:
Cytokine/Growth Factors
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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