aFGF (mouse), Mouse acidic Fibroblast Growth Factor Recombinant
Catalog # 90102-A
Size: 10 µg
Description: Recombinant murine
aFGF (acidic Fibroblast Growth Factor)
is a disulfide-linked monomer protein
consisting of 141 amino acids and
migrates as an approximately 16 kDa
protein under reducing and non-reducing
conditions. Native murine aFGF, generated
by the proteolytic removal of the signal
peptide and propeptide, has a calculated
mass of 16 kDa.
Source: Optimized DNA sequence
encoding murine acidic Fibroblast
Growth Factor mature chain was
expressed in E. coli.
Formulation: Lyophilized from a 0.2 μm
filtered solution containing 2.5% glycine,
0.5% sucrose, 0.01% Tween-80, 5 mM
glutamic acid, pH 4.5.
Reconstitution: Reconstitute at 0.1-1.0
mg/mL in distilled water. This solution
can then be diluted into other buffers. To
maximize product collection from vial
surface, vortex briefly and then spin
down to recollect the liquid.
Storage: The lyophilized protein is
stable for at least 2 years from date of
receipt when stored at -20°C. Upon
reconstitution, store in working aliquots
at 2 - 8°C for up to one month, or at
-20°C for up to six months, in the
presence of a carrier protein. Avoid
repeated freeze/thaw cycles.
Purity: >95%, as determined by SDS-
PAGE and HPLC.
Endotoxin Level: Endotoxin level was
found to be < 0.1 ng/μg (1EU/μg), using
the LAL gel clot method.
Biological Activity: The ED50 as
determined by the dose-dependent
stimulation of thymidine uptake by BaF3
cells expressing FGF receptors is < 10
ng/ml, corresponding to a specific
activity of ≥1 x 105 units/mg.
1. J. Clin. Pathol., Jan 2001; 54: 37 -
2. J. Biol. Chem., Apr 2000; 275: