CUL3/Rbx1, GST-tag

Catalog #: 80409
Size: 20 µg
$360
Species: Human
Host Species: Sf9 Insect Cell
MW: CUL3: 117 kDa; Rbx1: 12 kDa
Genbank #: CUL3: NM_003590; Rbx1: NM_014248
Tag(s): N-terminal GST-tag
a.a: CUL3: 2-768(end); Rnx1: 1-108(end)
Description:
Complex of human cullin-3 (CUL3) with human RING box protein 1 (Rbx1), also known as E3 ubiquitin-protein ligase RBX1. CUL3 (a.a. 2-768(end) with N-terminal GSTtag, 117 kDa) is co-expressed with Rbx1 (a.a. 1-108(end), 12 kDa) in a baculovirus infected Sf9 cell expression system. GenBank Accession Nos. are NM_003590 (CUL3) and NM_014248 (Rbx1).
UniProt
CUL3: Q13618; Rbx1: P62877
Synonym(s):
Cullin-3, RING box protein 1, RING box protein 1, E3 ubiquitin protein ligase 1
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 1.6 mM glutathione.
Format:
Aqueous buffer solution
Storage / Stability:
At least 6 months at -80°C
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Kaspar, J. et al., J. Biol. Chem. 2010; 285: 21349-21358

2. Kaspar, J. et al., J. Cell. Sci. 2012; 125: 1027-1038.

Warning(s):
Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
Scientific Category:
Ubiquitin E3 ligase
Product Type:
Recombinant Protein
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Product Datasheets

80409.pdf