CTLA4:B7-1[Biotinylated] Inhibitor Screening Assay Kit

Catalog #

72009

$1,125 *

Size: 96 reactions

Qty

Bulk/Custom

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Description

The CTLA4:B7-1[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CTLA4:B7-1 signaling. The key to this kit is the high sensitivity of detection of biotin-labeled B7-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CTLA4 is coated on a 96-well plate. Next, B7-1 is incubated with CTLA4 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

This product has been cited 4 times.

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Synonyms

Cytotoxic T-lymphocyte-associated protein 4, CD152, CTLA-4 Counter-Receptor, B7.1, CD80, activity assay kit, Strep HRP, streptavidin, CTLA-4

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Product Data Gallery

Product Info

Storage and Usage

Citations4

Assay Kit Format

Chemiluminescent

Supplied As

This kit comes in a convenient 96-well format, with biotin-labeled B7-1, purified CTLA4, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.

Format

Catalog #	Component	Amount	Storage
71114	B7-1, Biotin-labeled	5 µg	-80°C	Avoid freeze/ thaw cycles!
71149	CTLA4	10 µg	-80°C
79742	Streptavidin-HRP	10 µl	+4°C
79311	3x Immuno Buffer 1	50 ml	-20°C
79728	Blocking Buffer 2	50 ml	+4°C
79670	ELISA ECL substrate A (transparent bottle)	6 ml	+4°C
79670	ELISA ECL substrate B (brown bottle)	6 ml	+4°C
79699	White 96-well microplate	1	+4°C

UniProt #

P33681

Background

The activation of naïve T cells requires two signals, the specific T cell receptor recognition of MHC/Antigen on the surface of the antigen-presenting cell (APC), and the binding of B7-1 (CD80) ligand on the APC with the CD28 receptor on the T cell surface. Conversely, binding of CTLA4 to B7-1 on the T-cell surface results in an inhibitory signal and prevents T-cell activation. CTLA4:B7-1 interaction is an important drug target for the regulation of the host’s response to cancer.

References

1. Linsley, P.S., et al. J. Exp. Med. 1991, 174(3): 561-569.
2. Hurwitz, A.A., et al. Canc. Res. 2000, 60: 2444-2448.

Storage/Stability

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol

Applications

This kit is useful for screening for inhibitors of B7-1 binding to CTLA4.

Shipping Temperature

-80°C (dry ice)

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt.

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Immunotherapy/Cell Surface Receptor/Single-pass Transmembrane Protein

Regulatory Status

Research Use Only