CRE/CREB Reporter (Luc) - Jurkat Cell Line (cAMP/PKA Signaling Pathway)
The CRE/CREB Reporter (Luc) – Jurkat Cell Line contains a firefly luciferase gene under the control of multimerized cAMP response element (CRE) stably integrated into Jurkat cells. Elevation of the intracellular cAMP level activates cAMP response element binding protein (CREB) to bind CRE and induces the expression of luciferase. This cell line is validated for response to stimulation by Forskolin.
Interested in screening and profiling inhibitors, blocking antibodies, or activators of CRE-mediated signaling pathways without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
- Forskolin (Sigma, #F3917)
- Assay Medium: Thaw Medium 2 (BPS Bioscience, #60184)
- Growth Medium 2E (BPS Bioscience, #79638)
- 96-well tissue culture treated white clear-bottom assay plate (Corning, #3610)
- One-Step luciferase assay system (BPS Bioscience, #60690)
- Luminometer
The cell line has been screened using the PCR-based Venor®GeM Mycoplasma Detection kit (Sigma-Aldrich, #MP0025) to confirm the absence of Mycoplasma species.
1. Montminy, M.R. et al. (1987) Binding of a nuclear protein to the cyclic-AMP response element of the somatostatin gene. Nature 328(6126): 175-178.
2. Fan Chung, K. (2006) Phosphodiesterase inhibitors in airways disease. Eur. J. Pharmacol. 533(1-3): 110-117.