c-Met, GST-tag Recombinant

Catalog #

40255

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human cMet also known as, c-MET, Met Proto-Oncogene, HGFR, SFR, and Hepatocyte Growth Factor Receptor, GenBank Accession No. X54559, a.a. 956-1390 with N-terminal GST tag, expressed in a Baculovirus infected Sf9 cell expression system. MW= 74 kDa.

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Synonyms

c-MET, cMET, Met Proto-Oncogene, HGFR, SFR, Hepatocyte Growth Factor Receptor, RCCP2, AUTS9, Scatter Factor Receptor

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥44%

Format

Aqueous buffer solution

Formulation

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol and 3 mM DTT.

74 kDa

Amino Acids

956–1390

Specific Activity

64 pmol/min/μg

Genbank #

X54559

UniProt #

P08581

Tag(s)

N-terminal GST-tag

References

1. Emaduddin M. et al., Proc. Natl. Acad. Sci. U.S.A. 105 (7):2358-2362 (2008).
2. Drebber U. et al., Oncol Rep. 19(6):1477-83 (2008).

Storage/Stability

At least 6 months at –80°C

Assay Conditions

40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 0.2 mM DTT using 0.1 mg/ml IGF-1R tide substrate and 20 mM ATP. Reaction was done at 30oC for 40 min. Amount of ATP transferred was measured using Kinase-Glo reagent from Promega.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Receptor Tyrosine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40255 Lot 120419.pdf

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c-Met, GST-tag Recombinant

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