cKit (V654A), His-tag Recombinant

Catalog #

40251

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human Kit (V654A) (GenBank Accession No. NM_000222), a.a. 544-976(end) with substitution of valine654 to alanine and N-terminal His-tag, MW=53.4 kDa, expressed in an Baculovirus infected Sf9 cell expression system.

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Synonyms

KIT, cKIT, c-KIT, PBT, CD117, Piebald Trait, SCFR, Mast/Stem Cell Growth Factor Receptor

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥82%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole

53.4 kDa

Amino Acids

544–976(end)

Specific Activity

89 pmol/min/μg

Unit Definition

One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosine substrate per minute at pH 7.4 and 30°C.

Genbank #

NM_000222

UniProt #

P10721

Tag(s)

N-terminal His-tag

References

1. Schumaker, J.A., et al. (2008) J. Clin. Pathol. 61:109-114.
2. Sun, J. et al. (2009) J. Biol. Chem. 284: 11039-11047.

Storage/Stability

>6 months at –80°C.

Assay Conditions

Enzyme reaction is conducted in a buffer containing 50 mM HEPES, pH 7.4, 3 mM MgCl₂, 3 mM MnCl₂, 1 mM DTT, 3 µM Na orthovanadate, 0.1 mM ATP, 30 µg/ml Poly (Glu:Tyr)4:1 substrate, and 4 µg /ml recombinant cKit. Assay was done with 0.2 mg/ml Poly-(Glu-Tyr) peptide using ADPGlo^® (Promega) conditions at 30°C for 35 minutes.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Receptor Tyrosine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40251_Lot120806-G.pdf

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cKit (V654A), His-tag Recombinant

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