PCSK9[Biotinylated]-LDLR Binding Assay Kit
The PCSK9[Biotinylated]-LDLR Binding Assay Kit is designed for screening and profiling purposes. PCSK9 is known to function as a negative regulator of hepatic low-density lipoprotein receptors (LDLRs) by binding to the LDLR ectodomain. The key to this kit is the high sensitivity of detection of biotin-labeled PCSK9 by streptavidin-HRP. Only a few steps are required for the assay. First, LDLR ectodomain is coated on a 96-well plate. Next, PCSK9 is incubated with LDLR on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, measured using a chemiluminescence reader.
*NOTE: As of November 2022, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.
Need us to run inhibitor screens or profile your compounds against PCSK9-LDLR? Check out our PCSK9 Screening Services.
This product has been cited 7 times.
- PBS (Phosphate Buffer Saline)
- Luminometer or plate reader capable of reading chemiluminescence
- Rotating or rocker platform
Catalog # | Name | Amount | Storage | |
71304 | PCSK9[Biotinylated]* | 10 µg | -80°C | Avoid multiple freeze/ thaw cycles |
71205 | LDLR* | 10 µg | -80°C | |
79742 | Streptavidin-HRP | 10 µl | +4°C | |
33298 | 3x PL-01 Assay Buffer | 50 ml | -20°C | |
79728 | Blocking buffer 2 | 50 ml | +4°C | |
79670 | ELISA ECL Substrate A (translucent bottle) | 6 ml | Room Temp | |
ELISA ECL Substrate B (brown bottle) | 6 ml | Room Temp | ||
79699 | White 96-well microplate | 1 | Room Temp |
*The concentration of the protein is lot-specific and will be indicated on the tube.
PCSK9 regulates circulating cholesterol homeostasis by binding to the ectodomain of liver low-density lipid receptors and promoting their degradation. PCSK9 binds to the low density lipoprotein receptor (LDLR), the very low density lipoprotein receptor (VLDLR), the apolipoprotein E receptor (LRP1/APOER), and the apolipoprotein receptor 2 (LRP8/APOER2).
1. Chan, J.C. et al. (2009). Proc. Natl Acad. Sci. USA, 106, 9820-9825.
2. Liang, H., et al. (2012) J. Pharmacol. Exp. Ther. 340 2289-236