MYLIP/IDOL, His-FLAG-tag

Catalog #: 71208
Size: 20 µg
$450
Species: Human
Host Species: Sf9 insect cells
MW: 51.7 kDa
Genbank #: NM_013262
Tag(s): N-terminal His-FLAG-tag
a.a: 2–445(end)
Background:
MYLIP is an E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Its activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibition of neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.
Description:
Human MYLIP, also known as Inducible Degrader of the LDL receptor (IDOL), GenBank Accession No. NM_013262, a.a. 2-445(end), with N-terminal His-FLAG-tag, MW=51.7 kDa, expressed in a baculovirus expression system.
UniProt
Q8WY64
Synonym(s):
MYLIP, IDOL, Myosin Regulatory Light Chain Interacting Protein, Inducible Degrader Of The LDL-Receptor, BZF1,
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 µg/ml FLAG peptide, and 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C

Application(s):
Useful for studying protein binding and screening small molecules for drug discovery.
Reference(s):
1. Santos PC., et al. Lipids Health Dis. 2012;11:83.
2. Sorrentino V., et al. Eur Heart J. 2013;34(17):1292-7.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Ubiquitin E3 Ligase, Other
Product Type:
Recombinant Protein
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Product Datasheets

71208_Lot130521-HF.pdf