LDLR, FLAG-Avi-Tag (Mouse)

Catalog #: 71222
Size: 50 µg
$425
Species: Mouse
Host Species: HEK293 cells
MW: 87 kDa
Genbank #: NM_010700
Tag(s): C-terminal FLAG-Avi-tag
a.a: 22-790 (end)
Background:
The low density lipoprotein receptor (LDLR protein) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. In case of HIV-1 infection, it functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the LDLR protein, inducing LDLR degradation.
Description:
Mouse low density lipoprotein receptor (LDLR), also known as FH, FHC, and LDLCQ2, GenBank Accession No. NM_010700, a.a. 22-790, with C-terminal Flag-tag, MW=87 kDa87, expressed in a HEK293 cell expression system. *Note: LDLR is heavily glycosylated, resulting in higher molecular weight.
Synonym(s):
FH, FHC, LDLR, LDLCQ2, low density lipoprotein receptor
Formulation:
40 mM Tris HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Storage / Stability:
At least 6 months at –80°C. Avoid freeze thaw cycles. Storing diluted protein is not recommended, if necessary, use a carrier protein (BSA 0.1 – 0.5%).
Application(s):
Useful for studying protein binding and screening small molecules for drug discovery.
Reference(s):
1. Holla, L., et al., BMC Cell Biol. 2007 Mar 1,8:9.
2. Qian, YW., et al., J Lipid Res. 2007 Jul,48(7):1488-98.
3. Fasano, T., et al., Athersclerosis. 2009 Mar,203(1):166-71.
Notes:
* LDLR is heavily glycosylated, resulting in higher molecular weight. The two bands shown correspond to differing states of glycosylation.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Cholesterol Related
Product Type:
Recombinant Protein
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Product Datasheets

71222 lot 130110-D.pdf