LDLR, Biotin-labeled

Catalog #: 71206
Size: 20 µg
Species: Human
Host Species: HEK293 cells
MW: 87.7 kDa
Genbank #: NM_000527
Tag(s): C-terminal FLAG and Avi tags
a.a: 22–788(end)
The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. In case of HIV-1 infection, it functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of LDLR, inducing LDLR degradation.
Human low density lipoprotein receptor (LDLR), also known as FH, FHC, and LDLCQ2, GenBank Accession No. NM_000527, a.a. 22-788(end), with C-terminal FLAG and Avi tags, MW=87.7 kDa*, expressed in an HEK293 cell expression system and enzymatically biotinylated using Avitag™ technology.
FH, FHC, LDLR, LDLCQ2, low density lipoprotein receptor
Assay Conditions:
Coat a plate with 100 ng of PCSK9. Add a 25 µL reaction mix (50 mM HEPES pH 7.4, 50 mM NaCl, 200 µM CaCl2, 0.05% Tween, 0.1% BSA, and up to 200 ng LDLR-biotin) to the coated wells and incubate for 2 hr. Add Streptavidin-HRP labeled antibody and incubate 1 hr. Finally, add HRP chemiluminescent substrates and read luminescence.
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Useful for studying protein binding and screening small molecules for drug discovery.
1. Holla, L., et al., BMC Cell Biol. 2007 Mar 1,8:9.
2. Qian, YW., et al., J Lipid Res. 2007 Jul,48(7):1488-98.
3. Fasano, T., et al., Athersclerosis. 2009 Mar,203(1):166-71.
* LDLR is heavily glycosylated, resulting in higher molecular weight. The two bands shown correspond to differing states of glycosylation.
Avoid freeze/thaw cycles
Scientific Category:
Cholesterol-Related Proteins
Product Type:
Recombinant Protein
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Product Datasheets

71206 Lot 140326-BD.pdf