Ceramide Kinase, FLAG-tag Recombinant

Catalog #

40617

$425 *

Size: 10 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Human ceramide kinase (CERK), GenBank Accession No. NM_022766, a.a. 1-537(end) with C-terminal FLAG-tag, MW= 60 kDa, expressed in a baculovirus expression system.

This product has been cited 4 times.

●

Synonyms

CERK, Ceramide Kinase, Lipid kinase 4, LK4, Acylsphingosine Kinase

●

Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations4

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥40%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% Glycerol and 100 μg/mL Flag peptide.

60 kDa

Amino Acids

1–537(end)

Specific Activity

≥90 pmole/min/µg

Unit Definition

A unit of ceramide kinase activity is defined as the amount of enzyme required to produce 1 pmole of ceramide-1-phosphate/min at 37°C.

Genbank #

NM_022766

UniProt #

Q8TCT0

Tag(s)

C-terminal FLAG-tag

References

1. Mitra,P. et al., FEBS Lett. 581 (4), 735-740 (2007)
2. Kim,T.J. et al., Biochem. Biophys. Res. Commun. 342 (2), 611-617 (2006)

Storage/Stability

>6 months at –80°C.

Assay Conditions

CERK activity was measured at 37°C for 25 minutes in a buffer (20 mM HEPES pH 7.2, 80 mM KCl, 3 mM CaCl₂, 10 mM MgCl₂, 2 mM DTT, 5 μM ATP) using a beta-Octylglucoside mixed micellar assay with 100 μM Ceramide (BPS# 40618). ATP reduction was detected using Kinase-Glo^® Luminescent Kinase Assay Platform (Promega, Madison, WI).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Lipids

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

●

Datasheets/Protocols

40617_lot_170313.pdf

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

● General Protocols

● FAQs

● Contact Technical Service

●

Ceramide Kinase, FLAG-tag Recombinant

PRODUCTS

SERVICES

RESOURCES

COMPANY