CD137L (4-1BB ligand), His-tag

Catalog #: 71189
Size: 100 µg
Species: Human
Host Species: HEK293
MW: 24 kDa
Genbank #: NM_003811
Tag(s): N-terminal His-tag
a.a: 50-254

Human CD137L, also known as 4-1BB ligand (4-1BBL), tumor necrosis factor ligand superfamily member 9, TNFSF9. GenBank Accession No. NM_003811, a.a. 50-254 with an N-terminal His-tag, expressed in a HEK293 cell expression system. MW = 24 kDa.

4-1BB ligand, 4-1BBL, tumor necrosis factor ligand superfamily member 9, TNFSF9
Endotoxin Level:
<1 EU/μg
Assay Conditions:
Reaction done according to CD137[Biotinylated]:CD137L Inhibitor Screening Assay Kit (Catalog # 72025) protocol. Buffer is PBS + 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. Coat protein was added at 4 deg 100 ng/well. Various concentrations of biotinylated CD137 (0-13.5 ng/ul, 0.5 ng/ul is the recommended kit condition) added for 2h at RT. Strep-HRP added for 1h at RT.
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C.
Useful as a positive control for western blotting
1. Langstein, J. et al., J. Immunol. 1998; 160(5): 2488-2494.
2. Salih, H.R., et al., 2000; J. Immunol. 165(5): 2903-2910.

Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 μg/ml in PBS + glycerol and stored at -80°C.
Scientific Category:
Immunotherapy & Cell Surface Receptor
Product Type:
Recombinant Protein
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