TRPC7-HEK293 Recombinant Cell Line

Catalog #: 90030
Size: 2 vials
$8,000
Species: Human
Genbank #: NM_020389
Description:
Stable, recombinant HEK293 cell line expressing human TRPC7 (transient receptor potential cation channel, subfamily C, member 7, accession number NM_020389).
UniProt
Q70T24
Synonym(s):
TRPC7, transient receptor potential cation channel, subfamily C, member 7
Supplied As:
Each vial contains ~1.5 X 106 cells in 1 ml of 10% DMSO.
Biological Activity:
N-terminal FLAG tagged human TRPC7 channel has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. The function of TRPC7 was characterized by calcium assay. In TRPC7-HEK293 cells, addition of methacholine, an activator of phospholipase C-linked muscarinic receptors, significantly activates TRPC7, resulting Ca2+ entry in the cells.
Host Cell line:
HEK293 cells
Mycoplasma Testing:
The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Instructions for use:
See cell line data sheet for detailed culturing and assay protocol.
Storage / Stability:

Store in liquid nitrogen immediately upon receipt.

Application(s):
•Monitor TRPC7 calcium channel activity
•Screen for activators or inhibitors of TRPC7 calcium channel
Reference(s):
1. Moran MM et al. (2011). Nat Rev Drug Discov. 10(8):601-620
2. Vassort G. and Alvarez J. (2009). Can. J. Physiol. Pharmacol. 87: 100-107
3. Lievremont J., et al. (2004). Am J Physiol Cell Physiol 287: C1709-C1716.
Notes:

Contact us for pricing.

License Disclosure:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Ion Channel
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

90030.pdf