TRPM2-HEK293 Recombinant Cell Line

Catalog #: 90331
Size: 2 vials
$7,875
Species: Human
Genbank #: NM_003307
Background:
TRPM2 channel belongs to the transient receptor potential channel (TRP) superfamily which is divided into seven main subfamilies. TRPM2 channel is a nonselective, calcium-permeable cation channel highly expressed in the brain, vascular smooth muscle, endothelial cells and immune cells. It is activated by oxidative stressors such as hydrogen peroxide, cyclic ADP ribose, and NADP, leading to an increase in the intracellular free calcium concentration and cell death. TRPM2 is a potential drug target in a variety of human diseases, including cardiovascular and neurodegenerative diseases.
Description:
Stable, recombinant HEK293 cell line expressing tetracycline-inducible human TRPM2 (transient receptor potential cation channel, subfamily M, member 2, accession number NM_003307).
UniProt
O94759
Synonym(s):
transient receptor potential cation channel, subfamily M, member 2, TRPM2, KNP3, previously TRPC7
Supplied As:
Each vial contains ~1.5 X 106 cells in 1 ml of 10% DMSO.
Biological Activity:
N-terminal FLAG tagged human TRPM2 channel has been stably integrated into HEK293 cells and its expression can be induced by tetracycline (doxycycline). The tetracycline-inducible expression of TRPM2 was confirmed by Western blotting. The function of TRPM2 was characterized by calcium assay. TRPM2-HEK293 cells were pre-treated with doxycycline to induce the expression of TRPM2, then stimulated with H2O2, and calcium influx was observed. The H2O2-induced calcium influx via TRPM2 could be blocked by N-(p-Amylcinnamoyl) anthranilic Acid (ACA), a TRP channel blocker.
Host Cell line:
HEK293 cells
Mycoplasma Testing:
The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Instructions for use:
See cell line data sheet for detailed culturing and assay protocol.
Storage / Stability:

Store in liquid nitrogen immediately upon receipt.

Application(s):
•Monitor TRPM2 calcium channel activity
•Screen for activators or inhibitors of TRPM2 calcium channel
Reference(s):
1. Moran, M.M., et al. (2011). Nat. Rev. Drug Discov. 10(8):601-620
2. Song, Y., et al. (2008) J. Biomol. Screen. 13(1):54-61
3. Fonfria, E., et al. (2004). Br. J. Pharmacol. 143(1):186-192.
Notes:

Contact us for pricing.

License Disclosure:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Ion Channel
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

90331.pdf