IKCA1 (KCNN4) - HEK293 Recombinant Cell Line

Catalog #: 90330
Size: 2 vials
Species: human
Genbank #: NM_002250
IKCA1 is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. The encoded protein may be part of the predominant calcium-activated potassium channel in T-lymphocytes.
Stable, recombinant HEK293 cell line expressing human IKCA1, also known as KCNN4 (Intermediate conductance calcium-activated potassium channel protein 4), IK1, hKCa4, and hSK4, GenBank Accession No. NM_002250.
IKCA1, KCNN4, Intermediate conductance calcium-activated potassium channel protein 4, IK1, hKCa4, hSK4
Supplied As:
Each vial contains ~2 X 106 cells in 1 ml of 10% DMSO.
Biological Activity:
N-terminal FLAG-tagged human IKCA1 has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. Functional analysis of IKCA1 channel in the stable recombinant HEK293 was accomplished by using the membrane potential–sensitive fluorescence dye DiBAC4(3). When IKCA1-expressed HEK293 cells were stimulated with calcium-specific ionophore ionomycin to raise the intracellular level of Ca2+, IKCA1 channel was activated, leading to hyperpolarization and a decrease in cell fluorescence in the functional fluorescence assay with DiBAC4(3). This decrease of cell fluorescence was blocked by the IKCA1 channel inhibitor, clotrimazole (CLT).
Host Cell line:
HEK293 cells
Mycoplasma Testing:
The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Growth Media:

Thaw Medium 1 (BPS Cat. #60187): MEM medium (Hyclone #SH30024.01) supplemented with 10% FBS (Invitrogen #26140-079), 1% non-essential amino acids (Hyclone #SH30238.01), 1 mM Na pyruvate (Hyclone #SH30239.01), 1% Penicillin/Streptomycin (Hyclone SV30010.01)

Growth Medium 1B (BPS Cat. #79531): Thaw Medium 1 (BPS Cat. #60187) plus 400 μg/ml of Geneticin (invitrogen #11811031) to ensure the recombinant expression is maintained. IKCA1-HEK293 cells typically exhibit a cell division time of ~24 hours.

Instructions for use:
See data sheet for detailed culturing and assay protocol.
Storage / Stability:
Store in liquid nitrogen immediately upon receipt.
• Drug compound screening for activators or inhibitors of IKCA1
• Functional assays for IKCA1
• Efficient antigen for mouse immunization

1. Ghanshani S et al. (2000). J Biol Chem. 275(47): 37137-37149.
2. Hoffman JF et al. (2003). PNAS 100(12): 7366-7371.
3. de-Allie FA et al. (1996). Br J Pharmacol. 117(3):479-487.
4. Terstappen GC et al. (2003). Neurosci Lett. 346(1-2):85-88.


License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Avoid freeze/thaw cycles
Scientific Category:
Ion Channel
Product Type:
Cell Line
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