PAI-1 Reporter (Luc) – Mv1 Lu Cell Line
PAI-1 Reporter (Luc)-Mv1 Lu cell line is designed for monitoring transforming growth factor β (TGF-β)-induced plasminogen activator inhibitor-1 (PAI-1) expression. Transforming growth factor-β (TGF-β) is a potent regulator of cellular differentiation, proliferation, migration, and protein expression.
PAI-1 Reporter (Luc) –Mv1 Lu cell line contains a firefly luciferase gene under the control of PAI-1 responsive elements stably integrated into Mv1 Lu (NBL-7) cells, showing TGF-β pathway response. This cell line is validated for the TGF-β response to the induction of PAI-1 gene expression through luciferase activity.
Interested in screening and profiling inhibitors, blocking antibodies, or activators of TGF-β signaling without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.
Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.
Human TGF-β1 (R+D Systems #240-B-002,)
Thaw Medium 1 (BPS Cat #60187)
Growth medium: Thaw Medium 1 (BPS Cat #60187) + G418 700 µg/ml.
Assay medium: DMEM medium (Hyclone, #SH30243.01) + 1% BSA + 1% Pen/Strep
96-well tissue culture treated white clear-bottom assay plate (Corning, #3610)
ONE-STEP™ Luciferase Assay System (BPS Cat #60690) or other luciferase reagents for measuring firefly luciferase activity
Luminometer
Abe, M. et al. (1994) An assay for transforming growth factor- β using cells transfected with a plasminogen activator inhibitor-1 promoter-luciferase construct. Analytical Biochemistry 216:276-584.
Khan, S. et al. (2012) Quantification of active and total transforming growth factor-β levels in serum and solid organ tissues by bioassay. BMC Research Notes 5: 636