SLC5A5 – HEK293 Recombinant Cell line

Catalog #: 90333
Size: 2 vials
$7,875
Background:
Iodine transport is essential for the production of thyroid hormone (T4). Sodium Iodide Symporter SLC5A5 (NIS) is a sodium-dependent transporter of iodide into thyroid follicular cells and other peripheral tissues to support T4 synthesis [1]. Lack of iodide transport can result in hypothyroidism and can manifest itself in several disease states like goiter formation and weight gain. SLC5A5 also plays an important role in hyperthyroidism (an excessive production of T4). Expression and activity of SCL5A5 is altered in several forms of cancer. Inhibitors of SLC5A5 have potential therapeutic benefit for the regulation of iodine overload induced by amiodarone or other thyromimetics that can result in hyperthyroidism [2].
Description:
Stable recombinant HEK293 cell line expressing human SLC5A5 (UniProt # Q92911) with a C-terminal FLAG- tag. SLC5A5 is a sodium-dependent iodide transporter and is a member of the sodium glucose co-transporter family. The expression of SLC5A5 is confirmed by Western blotting. This cell line can be used to evaluate agents that may affect iodide transport.
UniProt
Q92911
Host Cell line:
HEK293 cells
Growth Media:

Thaw Medium 1 (BPS Cat. #60187): MEM medium (Hyclone #SH30024.01) supplemented with 10% FBS (Invitrogen #26140-079), 1% non-essential amino acids (Hyclone #SH30238.01), 1 mM Na pyruvate (Hyclone #SH30239.01), 1% Penicillin/Streptomycin (Hyclone SV30010.01)

Growth Medium 1B (BPS Cat. #79531): Thaw Medium 1 (BPS Cat. #60187) plus 400 µg/ml of Geneticin (Life Technologies #11811031).

Format:

Each vial contains ~1.5 X 106 cells in 1 ml of 10% DMSO.

Instructions for use:
See cell line data sheet for detailed culturing and assay protocol.
Storage / Stability:
Immediately upon receipt, store in liquid nitrogen.
Application(s):
• Drug compound screening

• Functional assays

Reference(s):

1. Dohán et al., (2003) The sodium/iodide Symporter (NIS): characterization, regulation, and medical significance. Endocrine Reviews 24(1):48-77.

2. Lindenthal et al., (2009) Characterization of NIS inhibitors. Journal of Endocrinology 200: 357–365.

3. Waltz et al., (2010) A nonradioactive iodide uptake assay for sodium iodide symporter function. Analytical Biochemistry 396(1): 91-5.

Notes:
License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Ion Channel
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

90333.pdf