NET – CHOK1 Recombinant Cell Line

Catalog #: 60557
Size: 2 vials

NET is a monoamine transporter responsible for the sodium-chloride (Na+/Cl−)-dependent reuptake of extracellular norepinephrine (NE), also known as noradrenaline. NET can also reuptake extracellular dopamine (DA). The reuptake of these two neurotransmitters is essential in regulating concentrations in the synaptic cleft. NETs, along with the other monoamine transporters, play a key role in depression and neurodegenerative diseases (NDD) such as Alzheimer's and Parkinson's disease. NET is the target of many antidepressants and recreational drugs. In addition, an overabundance of NET is associated with ADHD.

Recombinant CHOK1 cell line expressing human norepinephrine transporter (NET), Genbank accession number NM_001043.
Materials Required But Not Provided:

• Neurotransmitter Transporter Uptake Assay Kit (Molecular Devices #R6138) 
• HBSS buffer (1×) (Hyclone #SH30588.01).
Thaw Medium 3 (BPS Cat. #60186)
Growth Medium 3B (BPS Cat. #79529)

NET, CHO, NET Cell Line, CHOK1, CHO-K1
Host Cell line:
Mycoplasma Testing:
The cell line has been screened using the PCR-based Venor®GeM Mycoplasma Detection kit (Sigma-Aldrich, #MP0025) to confirm the absence of Mycoplasma species.
Growth Media:

Thaw Medium 3 (BPS Cat. #60186): Ham’s F-12 medium (Hyclone # SH30526.01) supplemented with 10% FBS (Life technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone SV30010.01).

Growth Medium 3B (BPS Cat. #79529): Thaw Medium 3 (BPS Cat. #60186) plus 500 μg/ml of Hygromycin B (Life Technologies # 10687-010) to ensure the recombinant expression is maintained.

Storage / Stability:
Immediately upon receipt, store in liquid nitrogen.
Monitor norepinephrine uptake activity and screen for activators or inhibitors of NET activities in a cellular context.

1. Zhou, J., Drugs Future. 2004; 29(12): 1235–1244.

2. Jorgensen, S., et al., Journal of Neuroscience methods. 2008; 169: 168-176.


License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Scientific Category:
Product Type:
Cell Line
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