Nav1.8/β2 - HEK293 Recombinant Cell Line

Catalog #: 60521
Size: 2 vials
$8,000
Species: Human
Host Species: HEK293 Cells, tetracycline-inducible
Genbank #: NP_006505.2, NM_004588
Background:
Nav1.8 is a voltage gated type X, alpha subunit sodium channel which in humans is encoded by the SCN10A gene. It is expressed in nociceptors and has been proposed as a target for the development of new analgesics. Mice deficient in Nav1.8 have deficits in sensing inflammatory pain (initiated by tissue damage/inflammation) and visceral pain (initiated by damage or injury to internal organs) but not neuropathic pain. Native sodium channels are complexes composed of the pore-forming α subunit and an auxiliary β subunit. The sodium channel β2 subunit, encoded by the SCN2B gene, increases current amplitude and surface expression of sodium ion channels.
Description:
Stable recombinant HEK293 cell line expressing tetracycline-inducible human Nav1.8 (Genbank #NP_006505.2) fused to Green Fluorescent Protein (GFP) [Ex. ~395 nm, 475 nm; em ~510 nm] and the human sodium channel β2 subunit (Genbank #NM_004588; SCN2B). Nav1.8 is also known as tetrodotoxin-resistant voltage-gated sodium channel type X (SCN10A).
Synonym(s):
NAV
Host Cell line:
HEK293 Cells
Mycoplasma Testing:
The cell line is confirmed for absence of Mycoplasma species using the PCR-based VenorGeM® Mycoplasma Detection kit (Sigma Aldrich).
Growth Media:

Thaw Medium 4 (BPS Cat. #60181): DMEM/F12 (1:1) (Hyclone # SH30271.01) supplemented with 10% FBS (Life Technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone # SV30010)

Growth Medium 4B (BPS Cat. #79554) : Thaw Medium 4 (BPS Cat. #60181) plus 10 µg/ml Blasticidin (Life Technologies # R210-01), 200 µg /ml Zeocin (Invivogen # ant-zn-1p), and 100 µg/ml Hygromycin B (Hyclone #SV30070.01)

Cells should be grown at 37°C with 5% CO2 using Growth Medium 4B.

Format:
Each vial contains ~1 X 106 cells in 10% DMSO solution
Instructions for use:
See product datasheet for detailed protocol.
Storage / Stability:
Immediately upon receipt, store in liquid nitrogen.
Application(s):
Drug compound screening. Functional assays. Efficient antigen for mouse immunization
Reference(s):

1. Catterall, W.A. et al. Pharmacol. Rev. 57 (4): 397–409 (2005).
2. Muzny, D.M., et al. Nature 440:1194-1198 (2006).
3. Wilson, D.S., et al. Protein Expression and Purification 23 (3): 440–446 (2001).

Notes:

Contact us for pricing.

License Disclosure:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Amino Acid Sequence:
A synthetic codon-optimized DNA sequence encoding human Nav1.8 (SCN10A gene) with C-terminal GFP-tag and C-terminal Streptavidin-Binding Peptide (SBP)-tag, and the SCN2B gene encoding human β2 subunit with C-terminal FLAG-tag are stably integrated in tetracycline-inducible HEK293 cells.
Scientific Category:
Ion Channel
Product Type:
Cell Lines
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

60521.pdf