TRPC3-HEK293 Recombinant Cell Line

Catalog #: 90130
Size: 2 vials
$7,875
Species: Human
Genbank #: NM_003305
Background:
TRPC3 channel belongs to the transient receptor potential channel (TRP) superfamily which is divided into seven main subfamilies. TRP channels share a similar architecture of six-transmembrane domains and function as tetramers. Most channels are permeable to calcium and monovalent cations (Vassort et al., 2009). Specifically, TRPC3 channel belongs to a canonical TRP (TRPC) subfamily that is involved in calcium entry. TRPC3 is activated through phospholipase C-linked receptors. When expressed in HEK293 cells, TRPC3 behaves as a receptor activated channel with constitutive activity that cannot be further increased by store depletion (Trebak et al., 2002).
Description:
Stable, recombinant HEK293 cell line expressing human TRPC3 (transient receptor potential cation channel, subfamily C, member 3, accession number NM_003305).
UniProt
Q13507
Synonym(s):
TRPC3, Transient Receptor Potential Cation Channel, Subfamily C, Member 3
Supplied As:
Each vial contains ~1 X 106 cells in 1 ml of 10% DMSO.
Biological Activity:
N-terminal FLAG tagged human TRPC3 channel has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. The function of TRPC3 was characterized by calcium assay. TRPC3 produces a constitutive Ca2+ entry in HEK293 cells. Addition of methacholine, an activator of phospholipase C-linked muscarinic receptors, significantly activates TRPC3.
Host Cell line:
HEK293 cells
Mycoplasma Testing:
The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Growth Media:

Thaw Medium 1 (BPS Cat. #60187): MEM medium (Hyclone #SH30024.01) supplemented with 10% FBS (Invitrogen #26140-079), 1% non-essential amino acids (Hyclone #SH30238.01), 1 mM Na pyruvate (Hyclone #SH30239.01), 1% Penicillin/Streptomycin (Hyclone SV30010.01)

Growth Medium 1B (BPS Cat. #79531): Thaw Medium 1 (BPS Cat. #60187) plus 400 μg/ml of Geneticin (invitrogen #11811031) to ensure the recombinant expression is maintained. TRPC3-HEK293 cells should exhibit a typical cell division time of 24 hours.

Instructions for use:
See data sheet for detailed culturing and assay protocol.
Storage / Stability:

Store in liquid nitrogen immediately upon receipt.

Application(s):
• Monitor TRPC3 calcium channel activity
• Screen for activators or inhibitors of TRPC3 calcium channel
Reference(s):

1. Albert A.P., Saleh S.N., and Large W.A. (2009). Current Medicinal Chemistry 16: 1158-1165.
2. Lievremont J., et al. (2004). Am J Physiol Cell Physiol 287: C1709-C1716.
3. Trebak M., et al. (2002). J. Biol. Chem. 277: 21617-21623.
4. Vassort G. and Alvarez J. (2009). Can. J. Physiol. Pharmacol. 87: 100-107.

Notes:

License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Ion Channel
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

90130.pdf