TMEM16A-HEK293 Recombinant Cell Line

Catalog #: 90230
Size: 2 vials
$7,875
Species: Human
Genbank #: NM_018043
Background:

Calcium-activated chloride channels (CaCCs) are major regulators in numerous physiological processes including sensory transduction, epithelial secrection, cardiac and neutonal excitation, and smooth muscle contraction.

TMEM16A (ANO1), a member of a family of putative plasma membrane proteins, is identified as a CaCC that is activated by intracellular Ca2+ and Ca2+-mobilizing stimuli. It has eight putative transmembrane segments without domains evidently involved in calcium regulation. The relative permeability of TMEM16A to monovalent anions is NO3- > I- > Br- > Cl- > F-.

Description:
Stable, recombinant HEK293 cell line expressing human TMEM16A (transmembrane protein 16A, also called anoctamin 1, calcium-activated chloride channel (ANO1), accession number NM_018043).
UniProt
Q5XXA6
Synonym(s):
TMEM16A, transmembrane protein 16A, anoctamin 1, calcium-activated chloride channel, ANO1
Supplied As:
Each vial contains ~1 X 106 cells in 1 ml of 10% DMSO.
Biological Activity:
N-terminal FLAG tagged human TMEM16A channel has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. The CaCC activity of TMEM16A was characterized by an assay based on halide-sensitive yellow fluorescent protein (YFP) mutant whose fluorescence is quenched by increasing halide concentration. When TMEM16A-expressed HEK293 cells were stimulated with ionomycin to raise the intracellular level of Ca2+, TMEM16A produced I influx in HEK293 that triggered the rapid decrease of fluorescence of transfected YFP mutant. The ionomycin-induced I influx through TMEM16A was blocked by niflumic acid, a CaCC channel blocker.
Host Cell line:
HEK293 cells
Mycoplasma Testing:
The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Growth Media:

Thaw Medium 1 (BPS Cat. #60187): MEM medium (Hyclone #SH30024.01) supplemented with 10% FBS (Invitrogen #26140-079), 1% non-essential amino acids (Hyclone #SH30238.01), 1 mM Na pyruvate (Hyclone #SH30239.01), 1% Penicillin/Streptomycin (Hyclone SV30010.01)

Growth Medium 1B (BPS Cat. #79531): Thaw Medium 1 (BPS Cat. #60187) plus 400 μg/ml of Geneticin (invitrogen #11811031) to ensure the recombinant expression is maintained.

Instructions for use:
See data sheet for detailed culturing and assay protocol.
Storage / Stability:
Store in liquid nitrogen immediately upon receipt.
Application(s):
• Drug compound screening for activators or inhibitors of TMEM16A
• Functional assays for TMEM16A
• Efficient antigen for mouse immunization
Reference(s):

1. Verkman A.S. and Galietta L.J.V. (2009) Cholide channels as drug targets. Nature Reviews 8: 153-171.
2. Caputo A. et al. (2008). Science 322: 590-594.
3. Yang Y.D. et al. (2008). Nature 455: 1210-1215.

Notes:

License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Ion Channel
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

90230.pdf