hERG (Kv11.1) - HEK293 Recombinant Cell line

Catalog #: 60619
Size: 2 vials
$1,375
Species: Human
Genbank #: NM_000238
Background:

hERG (human ether-a-go-go-related gene) encodes the alpha subunit of a potassium ion channel, Kv11.1. It contains six transmembrane α-helices with a re-entrant “poreloop” between the fifth and the sixth transmembrane helices. This ion channel is best known for its contribution to the electrical activity of the heart that coordinates the heart's beating. When this channel's ability to conduct electrical current across the cell membrane is inhibited or compromised, either by application of drugs or by rare mutations, it can result in a potentially fatal disorder called long QT syndrome. A number of clinically successful drugs in the market exhibit the potential to inhibit hERG, and create a concomitant risk of sudden death as a side-effect, which has made hERG inhibition an important off-target that must be avoided during drug development.

Description:
Recombinant HEK293 cell line expressing human ERG potassium channel (ether-a-gogo-related gene, also known as KCNH2 or Kv11.1, accession number NM_000238).
Synonym(s):
ERG, human ether-a-go-go-related gene, ether a go go ERG
Supplied As:
Each vial contains ~1.5 X 10^6 cells in 1 ml of 10% DMSO.
Biological Activity:
The channel has been validated for activity using a fluorescence-based assay that uses thallium influx as a surrogate indicator of potassium ion channel activity coupled with a thallium-sensitive fluorescent dye.
Host Cell line:
HEK293
Mycoplasma Testing:
The cell line has been screened using the PCR-based VenorGeM® Mycoplasma Detection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species.
Growth Media:

Thaw Medium 1 (BPS Cat. #60187): MEM medium (Hyclone #SH30024.01) supplemented with 10% FBS (Invitrogen #26140-079), 1% non-essential amino acids (Hyclone #SH30238.01), 1 mM Na pyruvate (Hyclone #SH30239.01), 1% Penicillin/Streptomycin (Hyclone SV30010.01).

Growth Medium 1B (BPS Cat. #79531): Thaw Medium 1 plus 400 μg/ml of Geneticin (Life Technologies #11811031).

Format:
Aqueous solution containing DMSO.
Instructions for use:
See data sheet for detailed culturing and assay protocol.
Storage / Stability:
Immediately upon receipt, store vials in liquid nitrogen.
Reference(s):

1. Beacham, D.W. et al. (2010). J. Biomol. Screen. 15(4):441-446

2. Murphy, S.M. et al. (2006) J. Pharmacol. Toxicol. Methods. 54(1):42-55

Notes:

License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Scientific Category:
Ion Channel
Product Type:
Recombinant Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

60619.pdf