Notch Signaling Pathway Notch1/CSL Reporter - HEK293 Recombinant Cell Line

Catalog #: 60652
Size: 2 vials
$1,375
Species: Human
Background:
The notch signaling pathway is important for cell-cell communication, which involves gene regulation mechanisms that control multiple cell differentiation processes during embryonic and adult life. Notch signaling also has a role in neuronal function, embryonic development, angiogenesis, cardiac homeostasis, and bone regeneration. Notch signaling is dysregulated in many cancers, and faulty notch signaling is implicated in many diseases including T-ALL (T-cell acute lymphoblastic leukemia) and Multiple Sclerosis.
Description:
The Notch CSL Reporter – HEK293 cell line contains the firefly luciferase gene under the control of Notch-response elements (CSL responsive elements) alone with expression construct for Notch1 E (NOTCH1 that has a deletion of the entire extracellular domain) stably integrated into HEK293 cells. Inside the cells, the Notch1 E can be cleaved by γ-secretase. The active Notch1 NICD is released to nucleus and induces the constitutive expression of luciferase reporter. The cell line is validated for the inhibition of the expression of luciferase reporter using a known inhibitor of the Notch signaling pathway.
Materials Required But Not Provided:

• Assay Medium: Thaw Medium 1 (BPS Cat. #60187)
Growth Medium 1A (BPS Cat. #79528)
• DAPT (Selleckchem # S2215): inhibitor of Notch pathway (γ-secretase  inhibitor). Prepare 10 mM of stock solution in DMSO.
• 96-well tissue culture plate or 96-well tissue culture-treated white clear-bottom assay plate
• ONE-Step™ Luciferase Assay System (BPS, Cat. #60690)
• Luminometer

Synonym(s):
Notch, NICD, PKA, cAMP, luciferase reporter
Supplied As:
Each vial contains ~1.5 X 106 cells in 1 ml of 10% DMSO.
Biological Activity:
The cell line is validated for the inhibition of the expression of luciferase reporter using a known inhibitor of the Notch signaling pathway.
Host Cell line:
HEK293 cells
Mycoplasma Testing:
The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Growth Media:

Thaw Medium 1 (BPS Cat. #60187): MEM medium (Hyclone #SH30024.01) supplemented with 10% FBS (Invitrogen #26140-079), 1% non-essential amino acids (Hyclone #SH30238.01), 1 mM Na pyruvate (Hyclone #SH30239.01), 1% Penicillin/Streptomycin (Hyclone SV30010.01).

Growth Medium 1A (BPS Cat. #79528): Thaw Medium 1 (BPS Cat. #60187) plus 100 µg/ml Hygromycin B (Thermo Fisher, Cat. #10687010) and 400µg/ml Geneticin®, G418 Sulfate (Thermo Fisher, Cat. # 10131035).

Format:
Aqueous solution containing DMSO.
Instructions for use:
See data sheet for detailed culturing and assay protocol.
Storage / Stability:

Store in liquid nitrogen immediately upon receipt.

Application(s):
• Monitor Notch signaling pathway activity.
• Screen for activators or inhibitors of the Notch signaling pathway.
Reference(s):

1. Lu, FM et al. (1996). Proc. Natl. Acad. Sci. USA 93(11): 5663-5667.
2. Kanungo, et al. (2008). J. Neurochem. 106: 2236.

Notes:

License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Cell Signaling Pathway
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

60652.pdf