NFAT Reporter (Luc) – Jurkat Recombinant Cell Line

Catalog #: 60621
Size: 2 vials
$1,375
Species: Human
Background:
The nuclear factor of activator T cells (NFAT) family of transcription factors plays an important role in immune response. T cell activation through the T cell synapse results in calcium influx. Increased intracellular calcium levels activate the calcium-sensitive phosphatase, calcineurin, which rapidly dephosphorylates the serine-rich region (SRR) and SP-repeats in the amino termini of NFAT proteins. This results in a conformational change that exposes a nuclear localization signal promoting NFAT nuclear import. In the nucleus, NFAT proteins cooperate with other proteins to bind to DNA.
Description:
The NFAT Reporter – Jurkat Cell Line contains a firefly luciferase gene under the control of the NFAT response element stably integrated into Jurkat cells. The NFAT Reporter cell line has been validated for response to thapsigargin, ionomycin, and phorbol 12-myristate 13-acetate (PMA). It is useful as a control cell line for other NFAT reporter cell lines expressing various immune checkpoint receptors.
Materials Required But Not Provided:
Synonym(s):
Nuclear factor of activated T cells; T cell
Supplied As:
Each vial contains ~2x10^6 cells in 1 ml of 10% DMSO.
Mycoplasma Testing:
The cell line has been screened using the PCR-based VenorGeM® Mycoplasma Detection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species.
Growth Media:

Thaw Medium 2 (BPS Cat. #60184): RPMI 1640 Medium (Thermo Fisher Cat. #11875093), with 10% heat-inactivated FBS, 1X Penicillin/ Streptomycin (Thermo Fisher, Cat. #10378016).

Growth Medium 2B (BPS Cat. #79530) Thaw Medium 2 (BPS Cat. #60184) and 1 mg/ml G418 (Thermo Fisher, Cat. #11811031).

Instructions for use:
See data sheet for detailed culturing and assay protocol
Storage / Stability:
Immediately upon receipt, store vials in liquid nitrogen.
Application(s):
• Monitor intracellular calcium levels. • Screen for activators or inhibitors of PKC/ Ca2+ pathway. • Screen for agonists or antagonists of T cell receptors. • Control for immune checkpoint NFAT reporter cell lines.
Reference(s):

1. Clipstone NA, Crabtree GR. Nature. 1992 Jun 25;357(6380):695-7.
2. Lyakh, L., et al. Mol Cell Biol. 1997 May;17(5):2475-84.

Application Reference(s):
1. Interleukin 2 Secretion by T Cells for Detection of Biologically Active Staphylococcal Enterotoxin Type E (2017)

Notes:

Contact us for pricing.

License Disclosure:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Immunotherapy
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

60621.pdf