NF-κB Reporter (Luc) - HCT116 Recombinant Cell Line

Catalog #: 60623
Size: 2 vials
Host Species: HCT-116

NF-kB luciferase reporter construct is stably integrated into the genome of HCT-116 cells. The
firefly luciferase gene is controlled by 4 copies of NF-κB response element located upstream of
the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors
bind to the DNA response elements to induce transcription of the luciferase gene.

The NF-κB-luciferase/HCT-116 cell line is suitable for monitoring the activity of NF-κB signaling
in response to stimulants such as the cytokines TNFalpha and IL-1β, pathogen-associated
molecular pattern (PAMP) (i.e. flagellin) or endogenous damage-associated molecular pattern
(DAMP) molecules (i.e. NOD1 ligand) (see application references). It is also suitable for
establishing cell-based screens for inhibitors that target specific NF-κB stimulating molecules.
This cell line can be further modified to allow investigation of downstream NF-κB activities as a
result of targeted genetic mutation(s).

NF-κB cell line, NFkappaB cell line, luciferase reporter, NF-kB cell line, NFkB cell line
Mycoplasma Testing:
This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, Cat. #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, Cat. #LT07-518) was used as a positive control.
Aqueous solution containing DMSO
Instructions for use:
See product datasheet
Storage / Stability:
Store in liquid nitrogen immediately upon receipt.

1. Samuel T (2014) Variable NF-B pathway responses in colon cancer cells treated with chemotherapeutic drugs. BMC Cancer 14: 599.
2. Arabi A (2012) Proteomic screen reveals Fbw7 as a modulator of the NF-B pathway. Nature Communication
3: 976. 3. Clemo NK (2008) BAG-1 is up-regulated in colorectal tumour progression and promotes colorectal tumour cell survival through increased NF-B activity. Carcinogenesis 29: 849.
4. Tukhvatulin AI (2011) An In Vitro and In Vivo Study of the ability of NOD1 Ligands to Activate the Transcriptional Factor NF-B. Acta Naturae 3: 77.


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License Disclosure:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Scientific Category:
Cell Signaling Pathway
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

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